PI-SceI

Type

Wild-Type

Target Sequence

-11	-10	-9	-8	-7	-6	-5	-4	-3	-2	-1	1	2	3	4	5	6	7	8	9	10	11
C	T	A	T	G	T	C	G	G	G	T	G	C	G	G	A	G	A	A	A	G	A

FASTA Sequence

Simple FASTA Numbered FASTA

Crystal Structure

PDB File

PyMOL File

Additional Information

Author: Barry Stoddard

Notes:
1. Host organism: Saccharomyces cerevisiae 2. Host gene: Vacuolar ATPase 3. Intein-associated endonuclease 4. Activity verified with in vitro cleavage experiments and structural analyses. 5. 6. Pubmed: http://www.ncbi.nlm.nih.gov/protein/CAA98762.1 RCSB: 1VDE, 1DFA, 1GPP, 1LWT, 1LWS, 1EFO 7. Target site listed for PI-SceI does not reflect total target site: additional contacts are made by auxiliary domains beyond the core LHE region

Date Added: 2012-01-02